A Zoospore Inoculation Method with Phytophthora sojae to Assess the Prophylactic Role of Silicon on Soybean Cultivars.
Identifieur interne : 001173 ( Main/Exploration ); précédent : 001172; suivant : 001174A Zoospore Inoculation Method with Phytophthora sojae to Assess the Prophylactic Role of Silicon on Soybean Cultivars.
Auteurs : Valérie Guérin [Canada] ; Amandine Lebreton [Canada] ; Erik E. Cogliati [Italie] ; Sue E. Hartley [Royaume-Uni] ; François Belzile [Canada] ; James G. Menzies [Canada] ; Richard R. Bélanger [Canada]Source :
- Plant disease [ 0191-2917 ] ; 2014.
Abstract
The objective of this study was to evaluate whether silicon (Si) amendments, known to have a prophylactic role against biotrophic and hemibiotrophic pathogens, could protect soybean against Phytophthora sojae. To fulfill this objective, the initial challenge was to develop a method of inoculation that reproduced the natural infection process while allowing regular Si feeding to the plants. In a first set of experiments, inoculation of P. sojae zoospores directly into hydroponic solutions led to reproducible infections and expected phenotypes when using 'Williams' (rps), 'L75-6141' (Rps1a), 'haro15' (Rps1k), and 'L77-1863' (Rps1b) soybean challenged to races 3 and 7 of P. sojae. This approach offers the advantage of testing simultaneously many soybean cultivars against different races of P. sojae in a controlled environment, and the expression of partial and root resistance. In a second set of experiments aimed at testing the effect of Si, our results clearly showed that Si amendments had a significant effect on disease reduction and plant yield. The effect was particularly noticeable when combined with a cultivar displaying a certain level of resistance to the disease. These results demonstrate a useful method of direct inoculation of soybean plants with P. sojae zoospores through a hydroponic system and show that Si amendments can represent an alternative method of control of P. sojae against soybean.
DOI: 10.1094/PDIS-01-14-0102-RE
PubMed: 30703877
Affiliations:
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Bélanger</name><affiliation wicri:level="4"><nlm:affiliation>Département de Phytologie, Université Laval.</nlm:affiliation><country>Canada</country><placeName><settlement type="city">Québec (ville)</settlement><region type="state">Québec</region></placeName><orgName type="university">Université Laval</orgName></affiliation></author></analytic><series><title level="j">Plant disease</title><idno type="ISSN">0191-2917</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The objective of this study was to evaluate whether silicon (Si) amendments, known to have a prophylactic role against biotrophic and hemibiotrophic pathogens, could protect soybean against Phytophthora sojae. To fulfill this objective, the initial challenge was to develop a method of inoculation that reproduced the natural infection process while allowing regular Si feeding to the plants. In a first set of experiments, inoculation of P. sojae zoospores directly into hydroponic solutions led to reproducible infections and expected phenotypes when using 'Williams' (rps), 'L75-6141' (Rps1a), 'haro15' (Rps1k), and 'L77-1863' (Rps1b) soybean challenged to races 3 and 7 of P. sojae. This approach offers the advantage of testing simultaneously many soybean cultivars against different races of P. sojae in a controlled environment, and the expression of partial and root resistance. In a second set of experiments aimed at testing the effect of Si, our results clearly showed that Si amendments had a significant effect on disease reduction and plant yield. The effect was particularly noticeable when combined with a cultivar displaying a certain level of resistance to the disease. These results demonstrate a useful method of direct inoculation of soybean plants with P. sojae zoospores through a hydroponic system and show that Si amendments can represent an alternative method of control of P. sojae against soybean.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30703877</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0191-2917</ISSN><JournalIssue CitedMedium="Print"><Volume>98</Volume><Issue>12</Issue><PubDate><Year>2014</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Plant disease</Title><ISOAbbreviation>Plant Dis</ISOAbbreviation></Journal><ArticleTitle>A Zoospore Inoculation Method with Phytophthora sojae to Assess the Prophylactic Role of Silicon on Soybean Cultivars.</ArticleTitle><Pagination><MedlinePgn>1632-1638</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PDIS-01-14-0102-RE</ELocationID><Abstract><AbstractText>The objective of this study was to evaluate whether silicon (Si) amendments, known to have a prophylactic role against biotrophic and hemibiotrophic pathogens, could protect soybean against Phytophthora sojae. To fulfill this objective, the initial challenge was to develop a method of inoculation that reproduced the natural infection process while allowing regular Si feeding to the plants. In a first set of experiments, inoculation of P. sojae zoospores directly into hydroponic solutions led to reproducible infections and expected phenotypes when using 'Williams' (rps), 'L75-6141' (Rps1a), 'haro15' (Rps1k), and 'L77-1863' (Rps1b) soybean challenged to races 3 and 7 of P. sojae. This approach offers the advantage of testing simultaneously many soybean cultivars against different races of P. sojae in a controlled environment, and the expression of partial and root resistance. In a second set of experiments aimed at testing the effect of Si, our results clearly showed that Si amendments had a significant effect on disease reduction and plant yield. The effect was particularly noticeable when combined with a cultivar displaying a certain level of resistance to the disease. These results demonstrate a useful method of direct inoculation of soybean plants with P. sojae zoospores through a hydroponic system and show that Si amendments can represent an alternative method of control of P. sojae against soybean.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Guérin</LastName><ForeName>Valérie</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Département de Phytologie, Université Laval, Québec, QC, G1V 0A6, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lebreton</LastName><ForeName>Amandine</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Département de Phytologie, Université Laval, Québec, QC, G1V 0A6, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cogliati</LastName><ForeName>Erik E</ForeName><Initials>EE</Initials><AffiliationInfo><Affiliation>Agroinnova, University of Turin, Via Leonardo da Vinci 44, 10095, Grugliasco (To), Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hartley</LastName><ForeName>Sue E</ForeName><Initials>SE</Initials><AffiliationInfo><Affiliation>Department of Biology, University of York, York, YO10 5DD, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Belzile</LastName><ForeName>François</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Département de Phytologie, Université Laval.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Menzies</LastName><ForeName>James G</ForeName><Initials>JG</Initials><AffiliationInfo><Affiliation>Agriculture and Agri-food Canada, 101 Route 100, Morden, MB, R6M 1Y5, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bélanger</LastName><ForeName>Richard R</ForeName><Initials>RR</Initials><AffiliationInfo><Affiliation>Département de Phytologie, Université Laval.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Dis</MedlineTA><NlmUniqueID>9882809</NlmUniqueID><ISSNLinking>0191-2917</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>2</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30703877</ArticleId><ArticleId IdType="doi">10.1094/PDIS-01-14-0102-RE</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li><li>Italie</li><li>Royaume-Uni</li></country><region><li>Québec</li></region><settlement><li>Québec (ville)</li></settlement><orgName><li>Université Laval</li></orgName></list><tree><country name="Canada"><region name="Québec"><name sortKey="Guerin, Valerie" sort="Guerin, Valerie" uniqKey="Guerin V" first="Valérie" last="Guérin">Valérie Guérin</name></region><name sortKey="Belanger, Richard R" sort="Belanger, Richard R" uniqKey="Belanger R" first="Richard R" last="Bélanger">Richard R. Bélanger</name><name sortKey="Belzile, Francois" sort="Belzile, Francois" uniqKey="Belzile F" first="François" last="Belzile">François Belzile</name><name sortKey="Lebreton, Amandine" sort="Lebreton, Amandine" uniqKey="Lebreton A" first="Amandine" last="Lebreton">Amandine Lebreton</name><name sortKey="Menzies, James G" sort="Menzies, James G" uniqKey="Menzies J" first="James G" last="Menzies">James G. Menzies</name></country><country name="Italie"><noRegion><name sortKey="Cogliati, Erik E" sort="Cogliati, Erik E" uniqKey="Cogliati E" first="Erik E" last="Cogliati">Erik E. Cogliati</name></noRegion></country><country name="Royaume-Uni"><noRegion><name sortKey="Hartley, Sue E" sort="Hartley, Sue E" uniqKey="Hartley S" first="Sue E" last="Hartley">Sue E. Hartley</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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